Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana
نویسندگان
چکیده
منابع مشابه
Molecular characterisation of two paralogous SPO11 homologues in Arabidopsis thaliana.
The Spo11 protein of yeast has been found to be covalently bound to double-strand breaks in meiosis, demonstrating a unique role of the protein in the formation of these breaks. Homologues of the SPO11 gene have been found in various eukaryotes, indicating that the machinery involved in meiotic recombination is conserved in eukaryotes. Here we report on SPO11 homologues in plants. In contrast t...
متن کاملMolecular characterisation of RecQ homologues in Arabidopsis thaliana.
Members of the RecQ family of DNA helicases are involved in processes linked to DNA replication, DNA recombination and gene silencing. RecQ homologues of various animals have been described recently. Here, for the first time for plants, we characterised cDNAs of all in all six different RecQ-like proteins that are expressed to different extents in Arabidopsis thaliana. Surprisingly, three of th...
متن کاملMolecular Characterisation of two Putative Photosynthetic Proteins in Arabidopsis thaliana
Photosynthesis is the biological process, by which photosynthetic organisms convert light energy and carbon dioxide into organic compounds. In plants, photosynthesis takes place in the chloroplast, and this organelle evolved from the ancestor of cyanobacteria by endosymbiosis. The chloroplast proteome contains thousands of proteins, of which most are encoded by the nuclear genome. Transcriptome...
متن کاملEvolutionary, Molecular and Genetic Analyses of Tic22 Homologues in Arabidopsis thaliana Chloroplasts
The Tic22 protein was previously identified in pea as a putative component of the chloroplast protein import apparatus. It is a peripheral protein of the inner envelope membrane, residing in the intermembrane space. In Arabidopsis, there are two Tic22 homologues, termed atTic22-III and atTic22-IV, both of which are predicted to localize in chloroplasts. These two proteins defined clades that ar...
متن کاملYeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.7.1548